C:\Users\Ana_CBGP\.opentrons\robot_settings.json not found. Loading defaults
C:\Users\Ana_CBGP\.opentrons\deck_calibration.json not found. Loading defaults
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WARNING(S):
	- Volume of tube in A3 of Cool_block_new 12 Tube Rack with No 1.5 mL on 5 is 1253uL and mixing volume should be 417.6666666666667uL, this volume cannot be picked and final mixing volume is going to be 300uL.
	- Volume of tube in B3 of Cool_block_new 12 Tube Rack with No 1.5 mL on 5 is 1253uL and mixing volume should be 417.6666666666667uL, this volume cannot be picked and final mixing volume is going to be 300uL.
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This warning(s) will not stop the proccess but should be inspected

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INFORMATION FILE
Data the users need to set the OT and perform the protocol
It is the output of the python file for the protocol when executed with the opentrons_simulate option

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GENERAL INFORMATION
This details are set by the user, this is only a remainder of some variables
	- Number of samples (colonies+controls): 58
	- Number of primer sets: 2
	- Number of total reactions: 116
	- Final volume of output labware (uL): 20.0
	- You have the replace tiprack option as False so there is no need of replacing none of the tipracks

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DECK LABWARE POSITIONS
This information will be provided by the OT App as well
	VWR Blueprint Depth 105 96 Well Plate 390 無 on 1
	VWR Blueprint Depth 105 96 Well Plate 390 無 on 2
	stacked_abgenes on 3
	stacked_abgenes on 4
	Cool_block_new 12 Tube Rack with No 1.5 mL on 5
	Opentrons 96 Tip Rack 300 無 on 6
	Opentrons 96 Tip Rack 20 無 on 7
	Opentrons 96 Tip Rack 20 無 on 8
	Opentrons Fixed Trash on 12

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COLDBLOCK REACTIVES POSITIONS
Reactives in each position of the coldblock(s) and their respective volume (minimum)

--> Coldblock in Slot 5
                     1                2                3  4
A        Water - 836uL  Primer 2 - 70uL  Set Reactives 1  -
B  Polymerase - 1392uL  Primer 3 - 70uL  Set Reactives 2  -
C      Primer 1 - 70uL  Primer 4 - 70uL                -  -

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Estimated protocol duration: 1h:3m
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WARNING: Protocol duration estimation is an experimental feature