FastQCFastQC Report
Wed 29 May 2024
all_reads_merged_quality.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameall_reads_merged_quality.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length360-2470
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGGTCAAAGATGCGCGGAACCAAGAGGTTTTTTCTATATAAAATTA12.127659574468085No Hit
AGAATTGATCTATATTGCGGACCCTAGAGGTCCCCTTTTTTATTTTAAAA12.127659574468085No Hit
TGCCTCTGTATCAATACTTTCCGGACCCTAGAGGTCCCCTTTTTTATTTT12.127659574468085No Hit
AGCCTGGTTCCAAGATTTGCGGAACCTAGAAGGTCCCCTTTTTTATTTTA12.127659574468085No Hit
ATAATTGGACCATTTAATTTCCGGACCTTCGAGGTCCCTTTCTTTTATTT12.127659574468085No Hit
AAACACGTTCAAGAATTTGCGGACCTAGAGGTCCCCTTTTTTATTTTAAA12.127659574468085No Hit
AGCTTCTGTTCTATTCTTTGCGGACCCTAGAGGTCCCCTTTTTTATTTTA12.127659574468085No Hit
TCGTTGGTCTGTGACGTCTCATGCTGGGCATCTCGTGCTGAGCGCGGCTC12.127659574468085No Hit
GAACCCTGGTCAAGATTTTCCGGAACCTAGAAGGTCCCCTTTTTTTATTT12.127659574468085No Hit
GGGTTATCAGGCATGACAACTGCATTTCATCTGATGCTGTGATGAATTTT12.127659574468085No Hit
AACTGTCTATTCTTTGCGGACCCTAGAGGTCCCCTTTTTTATTTTAAAAA12.127659574468085No Hit
GGAATTGACTGTATTGCGGACCCTAGAGGTCCCCTTTTTTATTTTAAAAA12.127659574468085No Hit
TGACCTTTATTATTGCGGACCTAGAGGTCCCCTTTTTTATTTTAAAAATT12.127659574468085No Hit
CATCCCTTGATATACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGAT12.127659574468085No Hit
GAATTCTCTACTATTGCGGACCCTAGAGGTCCCCTTTTTTATTTTAAAAA12.127659574468085No Hit
GGACCTTGGTTCATGAATTCGCCGGACCTTCGAGGTCCCTTTCTCTTTAT12.127659574468085No Hit
ATTATATGGACCATTTAATTTGCGGACCTTCGAGGTCCCTTTATTTTTAT12.127659574468085No Hit
TGTATCTGTATCATTGAATTCACCGGACCTTCGAAGGTCCCTTTATTTTT12.127659574468085No Hit
TGACCTGTTATTATTGCGGACCTAGAGGTCCCCTTTTTTATTTTAAAAAT12.127659574468085No Hit
ATAACTGGTCCAAAGATTTGCGGAACCTAGAAGGTCCCCTTTTTTTATTT12.127659574468085No Hit
GTGACTCTGTCTACTATTGCGGACCCTAGAGGTCCCCTTTTTTATTTTAA12.127659574468085No Hit
AGAATCTTGTTCAATTCTTTGCGGACCTAGAGGTCCCCTTTTTTTATTTT12.127659574468085No Hit
TCGGTTAGGTATGAGAACGATCTCATGCTGTGCGCGGTCTCGTGTCTGGA12.127659574468085No Hit
CCAACGCCAGATAGCGGGCAGCCTGCAATGGGCGTATATGGCGATTGCGC12.127659574468085No Hit
CTTCTCCTGGTACCATCGACTTCGCCGGTCCCTTCGAGGTCCCCTTTCTT12.127659574468085No Hit
AAACGTTTTCTATTATTGCGGACCTAGAGGTCCCTTTTTTATTTTAAAAT12.127659574468085No Hit
AGATATGTTCATTAATTTGCGGACCTAGAAGGTCCCCTTTTTTATTTTTA12.127659574468085No Hit
ATAACTGGTCAAAGAAGCGCGGAAGCAAGAGGCCTCTTTTTTATTTTAAA12.127659574468085No Hit
AGACACTGATCAATGAATTTGCGGACCTTAGAGGTCCCCTTTCTTTTTAT12.127659574468085No Hit
GTCCTCTGTATCAATGTCTTTGCGGACCCTTAGAGGTCCCCTTTTTTTTA12.127659574468085No Hit
CTTGATTTTCACTATTGCGGACCCTAGAGGTCCCCTTTTTTATTTTAAAA12.127659574468085No Hit
TTAATCTGTTCTATTATTGCGGACCCTAGAGGTCCCCTTTTTTATTTTAA12.127659574468085No Hit
AGACCTCTCTATTATTGCGGACCCTAGAGGTCCCCTTTTTTATTTTAAAA12.127659574468085No Hit
GGAATCTGTTCTATTCTTTGCGGACCCTAGAGGTCCCCTTTTTTATTTTA12.127659574468085No Hit
AGACTTATCTAGTATTGCGGACCCTAGAGGTCCCCTTTTTTATTTTAAAA12.127659574468085No Hit
CTTAATCTGTATCAATAATTTGCGGACCTAGAGGTCCCCTTTTTTTATTT12.127659574468085No Hit
TTAAACTTATTATTATTGCGGACCCTAGAGGTCCCCTTTTTTATTTTAAA12.127659574468085No Hit
AGAACTGTCTATTATTGCGGACCCTAGAGGTCCCCTTTTTTATTTTAAAA12.127659574468085No Hit
TGACTTGTTATATTGCGGACCCTAGAGGTCCCCTTTTTTATTTTAAAAAT12.127659574468085No Hit
AGACTGTCTCTATTGCGGACCCTAGAGGTCCCCTTTTTTATTTTAAAAAT12.127659574468085No Hit
AGAACCTGATCTATTCTTTGCGGACCCTAGAGGTCCCCTTTTTTATTTTA12.127659574468085No Hit
ATAACCTGGTCAATATTTGCGGAACCTAGAAGGTCCCCTTTTTTATTTTA12.127659574468085No Hit
ATTCCCTGGACCATTGAATTTGCGGACCTTCGAGGTCCCTTTCTTTTTAT12.127659574468085No Hit
AGAATTGATTATATTGCGGACCCTAGAGGTCCCCTTTTTTATTTTAAAAA12.127659574468085No Hit
AGATTGTTGTATTGCGGACCCTAGAGGTCCCCTTTTTTATTTTAAAAATT12.127659574468085No Hit
ATTAATGATGGACATGACAGTGGCGGAACCAAGAGGTCCTCTTTTTTATT12.127659574468085No Hit
GGCCCTCTGTTCAATTAATTGCCGGAACCTAAGAGGTTCCCTTTTTTATT12.127659574468085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)