-------------------------------------------------------------- -------------------------------------------------------------- INFORMATION FILE Data the users need to set the OT and perform the protocol It is the output of the python file for the protocol when executed with the opentrons_simulate option -------------------------------------------------------------- GENERAL INFORMATION This details are set by the user, this is only a remainder of some variables - Number total of selected colonies (all source plates): 89 - Number of total final plates: 3 - Final volume of output glycerol labware (uL): 50.0 - Final volume of output PCR labware (uL): 100.0 - Map of samples location with their respective identities is going to be stored at map_lib1_exampleColonieScreening.csv -------------------------------------------------------------- DECK LABWARE POSITIONS This information will be provided by the OT App as well VWR Blueprint Depth 105 96 Well Plate 390 無 on 1 VWR Blueprint Depth 105 96 Well Plate 390 無 on 2 VWR Blueprint Depth 105 96 Well Plate 390 無 on 3 VWR Blueprint Depth 105 96 Well Plate 390 無 on 4 Opentrons 15 Tube Rack with Falcon 15 mL Conical on 5 Opentrons 96 Tip Rack 300 無 on 6 Opentrons Fixed Trash on 12 -------------------------------------------------------------- 15 ML FALCON TUBERACK REACTIVES POSITIONS Reactives in each position of the coldblock(s) and their respective volume (minimum) Tuberack Opentrons 15 Tube Rack with Falcon 15 mL Conical on 5 --> Tuberack in Slot 5 1 2 3 4 5 A glycerol - 4460uL - - - - B water - 6685uL - - - - C - - - - - -------------------------------------------------------------- -------------------------------------------------------------- Estimated protocol duration: 0h:39m -------------------------------------------------------------- WARNING: Protocol duration estimation is an experimental feature